Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology)

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NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on ¹⁵N/¹³C-labeled proteins. Beginning with elementary quantum mechanics, a set of practical rules is presented and used to describe many commonly employed multi-dimensional, multi-nuclear NMR pulse sequences. A modular analysis of NMR pulse sequence building blocks also provides a basis for understanding and developing novel pulse programs. This text not only covers topics from chemical shift assignment to protein structure refinement, as well as the analysis of protein dynamics and chemical kinetics, but also provides a practical guide to many aspects of modern spectrometer hardware, sample preparation, experimental set-up, and data processing. End of chapter exercises are included to emphasize important concepts. Fundamentals of Protein NMR Spectroscopy not only offer students a systematic, in-depth, understanding of modern NMR spectroscopy and its application to biomolecular systems, but will also be a useful reference for the experienced investigator.

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Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology) Review

I'm a complete novice to NMR and this text provides a great introduction to the field with a focus on biological molecules. I've also read parts of Protein NMR Spectroscopy by John Cavanagh and I think Rule's book sets up the stage nicely for the reader to explore further by reading more detailed texts (Cavanagh) on particulars of the field. It's a relatively slim volume and not as intimidating in the mathematical modelling as some of the other books. If you need to understand an NMR phenomenon, read about it in this book first and it'll save you some time in developing an overall picture before looking at pages and pages of derivations in a dusty old book. I have Dr. Rule in part of my classes, I'm a first year grad student, and his amazing teaching ability in the classroom is reflected in the book. Highly recommended to those new to NMR along with Cavanagh's text. I wouldn't leave home without them.

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